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Biotech – Bioinfo Services
Biotechnology Services in India l RNA, DNA Technology
Cell or Molecular Biology forms an essential ground work in present day biological research. With experienced scientists and the most advanced level technologies, Lead Sciences Biotechnology Services presents aggregate of the molecular biology services to expedite your research in India.
- RNA Technology
- RNA Extraction and Purification
- Northern Blot
- Dot Blot and Slot Blot
- RT-PCR, Reverse-Transcriptase Polymerase Chain Reaction
- In situ Hybridization
- DNA Technology
- DNA Extraction and Purification
- Reporter Assays
- cDNA Purification for Microarrays
- Polymerase chain reaction (PCR)
- DNA Restriction Fragment Mapping
- DNA Subcloning
- cDNA Purification for Microarrays
- Library Screening and Gene Cloning
- Recombinant Protein Expression and Manufacturing
Cell Line Studies l Cell Lines Development India
Cell Lines act as in-vitro models to study the toxicity of compounds and for drug screening. Cell Lines are highly desirable as they help to provide the systems for direct admittance and tissue evaluation. Cell Cultures form a valuable tool to analyze clinical relevance usually related to diseases, studies of toxicity mechanisms and screening techniques. supports the feasibility for accessible studies of cellular mechanisms such as evaluation of therapeutic agents and promising drug targets that effectively treat a disease or dysfunction. They also form a source for the production of different cell types in vitro. Though cell lines serves as a possible alternative especially in cell transplant therapy, they display certain constraints in viability and stability. Nevertheless, certain rules must be considered to organize constant in-vitro functions.
Cell Based Services in India
- Flow Cytometry
- Confocal Microscope
- ROS Detection
- DNA Fragmentation
- Apoptosis
- Fluorescence Microscopy
- Maintenance of cell lines, testing cell viability
- Determination of IC50 for different drugs or compounds in different cell lines
- Cell survival assays
- Total cell lysate preparation
- Measurement of drug concentration
- SRB assay
- LDH assay
- Clonogenic assay
- P53 &P21 assay
- DNA topoisomerase I Assay
- DNA topoisomerase II Assay
- Cell cycle and DNA Damage analysis
- Caspase assays
- CDK analysis
- TUNEL assay
- BCL-2 assay
- Necrosis assay
BioChemistry and Enzymology Services
- Protein Production and Purification
- Protein Purification
- Differential Precipitation
- Chromatography
- HPLC
- Ion Exchange Chromatography
- Affinity Chromatography
- Stable Formulation Development
- Protein Analysis
- Protein Concentration
- Electrophoresis
- Binding Assays, Kd
- Amino Acid Analysis and Sequence
- Carbohydrate Analysis
- Solubility
- Enzyme Assays And Immunochemistry
- High Throughput Screening Assays (HTS)
- Enzyme Assays
- Enzyme and Protein Stability
Drug Designing Training Center in India
Lead Sciences is one of the leading best Drug Designing Coaching center in India and Service provider in Bio, Chemo-informatics, Genomics, Proteomics, Molecular Modeling and Drug Designing Structural Biology, BioIT, Microarray data analysis, and NGS data analysis are tailor made to suit customer requirements. Our value added services across the drug discovery chain, from discovery to development have been widely used by major pharmaceutical companies to boost their R&D efforts.
Bioinformatics Training Course Intro
- Introduction to bioinformatics and applications
- Biological databases
Genomics
- Genome annotation
- ORF detection
- Primer designing
- SNP-RFLP detection
- Contigs assembly
- Vector Construction
Proteomics
- Primary and secondary structure analysis
- Sequence analysis
- Phylogenetic tree analystis
- Evolutionary trace residues analysis
- Homology modeling
- Molecular dynamics simulation studies
- Active site Pocket prediction
Molecular Modeling Techniques:
- Molecular mechanics and quantum mechanics
- Forcefield method
- Minimization methods
- Conformational searching methods
Drug designing: Structure based Drug Designing
- Molecular docking studies
- Protein-Protein docking studies
- DNA-Ligand docking studies
- Scaffold replacement
- Multi-Fragment Search
Qsar
- 2d&3d qsar analysis
- Molecular descriptor
- Principle component analysis
- Multiple linear regression model
- COMFA and COMSIA studies
Phamacophore Mapping
- Receptor-based Pharmacophore mapping
- Ligand based Pharmacophore mapping
- Interaction Map Generation
Fragment based Drug Designing Training
- Fragment based drug designing
- Inside-out and Outside – approaches
- ADME and Topkat studies
Structure-Based Design Services
- Homology Modeling of drug targets
- Preparation of Protein and ligand molecules
- Active site Pocket prediction
- Protein-ligand docking
- DNA-ligand docking
- Computational analysis of docked pose
- Protein-Protein docking studies
- Virtual screening of small molecule libraries
Medicinal Chemistry Services / Organic Chemistry Services
Lead Sciences provides consulting services and strategic guidance in the drug design and discovery projects. Our staff are well qualified and deal with the below services.
- Creating compound candidate profiles
- Synthesizing new analogs
- Developing optimization strategies
- Improving compound properties
- Assessing drug-like properties of lead molecules
- Assessing the drug discovery progress
- IR analysis
- MASS Analysis
- FTIR Analysis
- LCMS Analysis
- NMR Analysis
- HPLC Analysis
- GC Analysis
- UV Analysis
- TLC Analysis (Thin Layer Chromatography Analysis)
- Column Chromatography & Purification Service
- HPTLC Analysis (High Performance Thin Layer Chromatography Analysis)
- Protein structure prediction
- Molecular dynamics simulation studies
- Molecular docking studies
- Qsar studies
- Fragment based drug designing services
- Pharmacophore mapping studies
- ADME and Toxicity studies
Pharmacology Services
In vivo Activity
- DLA/EAC induced ascitic tumor model in mice
- DLA induced solid tumor model in mice
In Vitro Activity
- Cytotoxicity assay by MTT/SRB/Trypan blue
- Clonogenic assay
- Apoptosis – Nuclear staining with DAPI
- Alkylating activity assay
- TUNEL assay
- Endonuclease assay
- DNA fragmentation analysis / DNA ladder assay
- Cancer specific gene expression analysis
- P53, BCl2, Capsase 3, VEGF, cyclin D1, cyclin B1, STAT3 etc.
Anti Diabetes Activity ( In vivo Activity )
- Alloxan induced diabetic rat model
- Streptozotocin induced diabetic rat model
- Glucose uptake studies in skeletal muscle cell lines (Non-radio labeled assay).
- Glucose uptake in isolated rat hemi diaphragm
- Gene expression studies (PPARγ, GLUT-4 etc)
- Alpha-Amylase inhibitory activity
- Alpha-galactosidase inhibitory activity
- Alpha-glucosidase activity
Hepatoprotective Activity / In vivo Activity
- Hepatoprotective study against selected toxicant in rats In Vitro Activity
- Hepatoprotective study against selected toxicant.
- Hepatoprotective study in isolated primary rat hepatocytes.
- Gene expression studies
Anti infective Activity In vivo Activity
- Different wound healing models in mice/rats
- Anti microbial activity by Zone of inhibition.
- Anti microbial activity by MIC determination
- Antibiotic sensitivity testing
- TLC bioautography
- In vitro anti viral studies
Other in vivo services
- Hypercholesterolemia
- Arthritis
- Osteoporosis
- Benign prostatic Hyperplasia
- Spermatogenesis
- Allergy
- Antitussive
- Psoriasis
Other services
- Antimicrobial
- Anti viral
- Cytotoxicity
- Cell proliferation
- Cancer
- Diabetes
- Hepatoprotection
- Obesity
- Immunomodulatory
- Inflammation
- Skin whitening
- Skin irritation (Dermal toxicity)
- Psoriasis
- Wound healing
- Genotoxicity
- Drug permeability
- Gene expression analysis
- Enzyme and Immunoassays (ELISA)
MicroBiology and Immunology Services
At Lead Sciences, we excel in microbiology services for quality control by providing a set of services for the Biopharmaceutical industry. Our capabilities categorize from estimation of microbial contamination to validation of antimicrobial activity. Our facilities fit the engineering requirements of microbiology testing and demanding cleanliness.
- Isolation of Nucleic acids from Microorganisms
- Isolation of plasmids
- Biochemical analysis of Microorganisms
- Antibiotic sensitivity test
- Minimum inhibition Concentration (MIC Studies)
- 16s rRNA sequencing
- Mutational studies
- Cloning and Expression
- Bacteria and yeast expression vectors
- Phylogenetic tree analysis
- Isolation and screening of industrially important microorganisms for enzyme production.
Immunology Services Include
- ELISA and Rocket Immuno electrophoresis
- Westernblot
- Northern Blot
- Southern Blot
- RadioImmune Assay (RIA)
- Epitope Mapping
- Immunoprecipitation
Medicinal Chemistry Services / Organic Chemistry Services
Lead Sciences Pre-clinical Services are designed to help you through the drug candidate selection process and to bridge the gap between the laboratory and clinical phase of research for pharmaceutical and biotechnology products..
Just as importantly, it has been successfully tried and tested by many clients who needed support during preclinical and clinical studies for regulatory submissions of investigative and new drugs. Working to the same high standards that underpin all of our services, our experts will help you evaluate the potential of a drug candidate to move into the more costly clinical phases of research. Through our preclinical laboratory services, we will analyze the initial pharmacokinetics and toxicokinetics of your investigational product.
In vivo Activity
- DLA/EAC induced ascitic tumor model in mice
- DLA induced solid tumor model in mice
In Vitro Activity
- Cytotoxicity assay by MTT/SRB/Trypan blue
- Clonogenic assay
- Apoptosis – Nuclear staining with DAPI
- Alkylating activity assay
- TUNEL assay
- Endonuclease assay
- DNA fragmentation analysis / DNA ladder assay
- Cancer specific gene expression analysis
- P53, BCl2, Capsase 3, VEGF, cyclin D1, cyclin B1, STAT3 etc.
Anti Diabetes Activity ( In vivo Activity )
- Alloxan induced diabetic rat model
- Streptozotocin induced diabetic rat model
- Glucose uptake studies in skeletal muscle cell lines (Non-radio labeled assay).
- Glucose uptake in isolated rat hemi diaphragm
- Gene expression studies (PPARγ, GLUT-4 etc)
- Alpha-Amylase inhibitory activity
- Alpha-galactosidase inhibitory activity
- Alpha-glucosidase activity
Hepatoprotective Activity / In vivo Activity
- Hepatoprotective study against selected toxicant in rats In Vitro Activity
- Hepatoprotective study against selected toxicant.
- Hepatoprotective study in isolated primary rat hepatocytes.
- Gene expression studies
Anti infective Activity In vivo Activity
- Different wound healing models in mice/rats
- Anti microbial activity by Zone of inhibition.
- Anti microbial activity by MIC determination
- Antibiotic sensitivity testing
- TLC bioautography
- In vitro anti viral studies
Other in vivo services
- Hypercholesterolemia
- Arthritis
- Osteoporosis
- Benign prostatic Hyperplasia
- Spermatogenesis
- Allergy
- Antitussive
- Psoriasis
Other services
- Antimicrobial
- Anti viral
- Cytotoxicity
- Cell proliferation
- Cancer
- Diabetes
- Hepatoprotection
- Obesity
- Immunomodulatory
- Inflammation
- Skin whitening
- Skin irritation (Dermal toxicity)
- Psoriasis
- Wound healing
- Genotoxicity
- Drug permeability
- Gene expression analysis
- Enzyme and Immunoassays (ELISA)
Analytical Chemistry
Analytical sciences employs numerous scientific disciplines such as biochemistry, physics, chemistry, engineering and mathematics. It deals with the measurement and eventually the metrology when the variables are well inferred and composed.
Analytical chemistry deals with the following disciplines.
- Structural analysis of various compounds.
- Qualitative analysis helps to identify and also determine the relative and absolute amounts of the groups, species,compounds, and elements in a certain sample.
Analytical Services Include
- LC-MS
- NMR 300 MHz
- FTIR (Quantitative and Qualitative)
- X-ray diffraction
- TGA,TMA
- DSC, SEM, EDAX, TRM
- HPLC and HPTLC
- UV, CHNS
- Mass spectrophotometry.
- Zeta Potential Analysis, IR
- Density testing
- Muffle furnace